Journal: Biomedicines

Article Title: Inhibition of AKT/GSK3β/CREB Pathway Improves the Responsiveness to AMPA Receptor Antagonists by Regulating GRIA1 Surface Expression in Chronic Epilepsy Rats

doi: 10.3390/biomedicines9040425

Figure Lengend Snippet: Primary antibodies used in the present study.

Article Snippet: p-CREB S133 , Rabbit , Novus biologicals (NB110-55727) , 1:5000 (WB).

Techniques:

Journal: Biomedicines

Article Title: Inhibition of AKT/GSK3β/CREB Pathway Improves the Responsiveness to AMPA Receptor Antagonists by Regulating GRIA1 Surface Expression in Chronic Epilepsy Rats

doi: 10.3390/biomedicines9040425

Figure Lengend Snippet: The effects of perampanel (PER) and GYKI 52466 (GYKI) on total Ca 2+ /cAMP response element-binding protein (CREB) expression and its S133 phosphorylation in chronic epilepsy rats. ( A ) Representative images for Western blot of CREB and its S133 phosphorylation level in the hippocampal tissues. ( B – D ) Quantifications of CREB (B), p-CREB S133 ( C ), and p-CREB/CREB ratio ( D ) in the hippocampal tissues. Open circles indicate each individual value. Horizontal bars indicate mean value. Error bars indicate SEM ( * ,# p < 0.05 vs. control (Cont) and vehicle (Veh)-treated animals, respectively; one-way ANOVA with post hoc Bonferroni’s multiple comparison).

Article Snippet: p-CREB S133 , Rabbit , Novus biologicals (NB110-55727) , 1:5000 (WB).

Techniques: Binding Assay, Expressing, Phospho-proteomics, Western Blot, Control, Comparison

Journal: Biomedicines

Article Title: Inhibition of AKT/GSK3β/CREB Pathway Improves the Responsiveness to AMPA Receptor Antagonists by Regulating GRIA1 Surface Expression in Chronic Epilepsy Rats

doi: 10.3390/biomedicines9040425

Figure Lengend Snippet: The effects of 3CAI co-treatment with perampanel (PER) and GYKI 52466 (GYKI) on expression levels of GSK3β, CREB, and PICK1 and the phosphorylation levels of GSK3β and CREB in non-responders. ( A ) Representative images for Western blot of total GSK3β, CREB, and PICK1 levels and the phosphorylation levels of GSK3β and CREB. ( B – H ) Quantifications of GSK3β ( B ), p-GSK3β S9 ( C ), p-GSK3β S9/GSK3β ratio ( D ), CREB ( E ), p-CREB S133 ( F ), p-CREB S133/CREB ratio ( G ), and PICK1 ( H ) in the hippocampal tissues. Open circles indicate each individual value. Horizontal bars indicate mean value. Error bars indicate SEM ( *, # p < 0.05 vs. control (Cont) and vehicle (Veh)-treated animals, respectively; one-way ANOVA with post hoc Bonferroni’s multiple comparison).

Article Snippet: p-CREB S133 , Rabbit , Novus biologicals (NB110-55727) , 1:5000 (WB).

Techniques: Expressing, Phospho-proteomics, Western Blot, Control, Comparison

Journal: Frontiers in Immunology

Article Title: Granulocytic Myeloid-Derived Suppressor Cell Exosomal Prostaglandin E2 Ameliorates Collagen-Induced Arthritis by Enhancing IL-10 + B Cells

doi: 10.3389/fimmu.2020.588500

Figure Lengend Snippet: G-MDSC exosomal PGE2 promotes IL-10 + B cells (A) COX-2 expression in G-MDSCs was detected after treatment with the COX-2 inhibitor celecoxib. (B) The ratio of COX2 and GAPDH in each group was statistically analyzed. (C) The level of PGE2 in the G-MDSC culture supernatant treated with celecoxib was detected by ELISA. (D) The level of PGE2 in the G-exo treated with celecoxib was detected by ELISA. (E) IL-10–producing B cells after treatment with celecoxib-treated G-exo in the present of LPS were analyzed by flow cytometry. (F) The level of IL-10 in culture supernatant after treatment with celecoxib-treated G-exo was detected by ELISA. (G) The p-GSK-3β, T-GSK-3β, p-CREB, and T-CREB were detected by Western blot analysis. (H) The ratio of p-CREB and T-CREB in each group was statistically analyzed. (I) The ratio of p-GSK-3β and T-GSK-3β in each group was statistically analyzed. Bar graphs show the means ± SD. *P < 0.05; **P < 0.01; ***P < 0.001; ns: indicates no significance.

Article Snippet: The membranes were then incubated with specific rabbit antibodies against phosphorylated (p)-GSK-3β (Santa Cruz Biotechnology, Texas, USA ) , total (t)- GSK-3β (Wanleibio, Shenyang, China), phosphorylated (p)-cAMP response element binding protein (CREB) (Wanleibio, Shenyang, China) and total (t)-CREB (Wanleibio, Shenyang, China) followed by an incubation with the secondary HRP-conjugated goat anti-rabbit IgG (CST, Danvers, MA, USA) according to manufacturer’s protocol.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Flow Cytometry, Western Blot

Journal: Frontiers in Immunology

Article Title: Granulocytic Myeloid-Derived Suppressor Cell Exosomal Prostaglandin E2 Ameliorates Collagen-Induced Arthritis by Enhancing IL-10 + B Cells

doi: 10.3389/fimmu.2020.588500

Figure Lengend Snippet: Schematic image demonstrating that Granulocytic myeloid-derived suppressor cell exosomal prostaglandin E2 ameliorates collagen-induced arthritis by inducing IL-10 + B cells production via affecting GSK-3β and CREB phosphorylation.

Article Snippet: The membranes were then incubated with specific rabbit antibodies against phosphorylated (p)-GSK-3β (Santa Cruz Biotechnology, Texas, USA ) , total (t)- GSK-3β (Wanleibio, Shenyang, China), phosphorylated (p)-cAMP response element binding protein (CREB) (Wanleibio, Shenyang, China) and total (t)-CREB (Wanleibio, Shenyang, China) followed by an incubation with the secondary HRP-conjugated goat anti-rabbit IgG (CST, Danvers, MA, USA) according to manufacturer’s protocol.

Techniques: Derivative Assay, Phospho-proteomics